Position overview
Partial
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Fields of study
Plant Sciences, Biology
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Country
Netherlands
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Deadline
2026-10-08
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Supervisor
Not specified
- Institute
- Source
- Website of the institution
- Email to apply
This open PhD listing is hosted by University of Amsterdam (UvA), based in Netherlands. The advertised research focus covers Plant Sciences, Biology. The application deadline shown for this call is 2026-10-08. Funding notes on this listing: Partial. Key details, funding information, how to apply and a fuller description of the position are collected below from the source vacancy — verify dates and eligibility on the official apply page before submitting an application.
Key details
- Host institution: University of Amsterdam (UvA)
- Location: University of Amsterdam (UvA), Spui 21, Amsterdam, Netherlands
- Application deadline: October 9, 2026
Funding
Not funded by a EU programme
Funding level (from listing): partial or not via EU framework — confirm on the official call.
How to apply
- Application portal / instructions: Official application link.
- Context on Euraxess: Original listing.
About the position
About the Project
Plants must continuously perceive, integrate and interpret a vast number of simultaneous environmental signals—such as drought, soil salinity and pathogen attack—and convert this complex input into coordinated responses. While responses to individual signals are well understood, the molecular mechanisms by which dedicated proteins enable rapid signal integration remain largely unexplored. This PhD position is the second appointment in an NWO VIDI-funded project led by Dr. André Kuhn, based in the Plant Cell Biology group at the University of Amsterdam (UvA). The project investigates the biochemical and cell-biological basis of signal integration via B4-RAF kinase signalling complexes, which are activated rapidly by diverse environmental stimuli and assemble into distinct subcellular puncta—suggesting the formation of signalosomes that coordinate early cellular responses. Research combines plant molecular biology, protein biochemistry, functional proteomics, quantitative live-cell imaging and genetics.
Research Activities
- Identify candidate B4-RAF signalosome components by immunoprecipitation coupled to tandem mass spectrometry (IP–MS/MS), building on existing proximity-labelling datasets.
- Validate protein interactions and co-localisation using FRET-FLIM, co-immunoprecipitation and live-cell confocal imaging.
- Generate and characterise Arabidopsis loss-of-function and fluorescent reporter lines.
- Apply microfluidics-coupled live-cell imaging to study the spatiotemporal dynamics and subcellular localisation of B4-RAF complexes under distinct environmental conditions.
- Integrate proteomics, imaging and genetic datasets to develop mechanistic models of rapid plant signal integration.
Tasks and Responsibilities
Location
University of Amsterdam (UvA), Spui 21, Amsterdam, Netherlands